Use of Urine with Nested PCR Targeting the Flagellin Gene ( fliC ) for Diagnosis of Typhoid Fever
نویسندگان
چکیده
منابع مشابه
Early diagnosis of typhoid fever by nested PCR for flagellin gene of Salmonella enterica serotype Typhi
BACKGROUND & OBJECTIVES Typhoid fever caused by Salmonella Typhi continues to be a major health problem in spite of the use of antibiotics and the development of newer antibacterial drugs. Inability to make an early laboratory diagnosis and resort to empirical therapy, often lead to increased morbidity and mortality in cases of typhoid fever. This study was aimed to optimize a nested PCR for ea...
متن کاملTargeting of putative fimbrial gene for detection of S. Typhi in typhoid fever and chronic typhoid carriers by nested PCR.
INTRODUCTION It is important to identify Salmonella Typhi infection quickly to treat acute fever patients and to prevent transmission by chronic typhoid carriers; therefore, a very specific and sensitive diagnostic technique is highly desirable, especially in endemic areas. The objective of this study was to develop a PCR protocol targeting the putative fimbrial staA gene of S. Typhi. This is a...
متن کاملthe use of appropriate madm model for ranking the vendors of mci equipments using fuzzy approach
abstract nowadays, the science of decision making has been paid to more attention due to the complexity of the problems of suppliers selection. as known, one of the efficient tools in economic and human resources development is the extension of communication networks in developing countries. so, the proper selection of suppliers of tc equipments is of concern very much. in this study, a ...
15 صفحه اولEarly Diagnosis of Typhoid By PCR For FliC-d Gene of Salmonella Typhi in Patients Taking Antibiotics.
OBJECTIVE To compare PCR (Polymerase Chain Reaction) with blood culture, typhi-dot and Widal test for the diagnosis of typhoid in patients taking antibiotics. STUDY DESIGN Cross-sectional, comparative study. PLACE AND DURATION OF STUDY National University of Sciences and Technology, Islamabad, Pakistan, from April 2013 to August 2014. METHODOLOGY One hundred and five patients were include...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2012
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00031-12